2011;
PLATELET ATP AND L-ARGININE-ASYMMETRICAL DIMETHYLARGININE RATIO IN VIBRATIONAL TRAINING VERSUS AEROBIC TRAINING OF PARKINSON’S DISEASE
PATIENTS. A PRELIMINARY STUDY
Jahr: 2011
P. Bongioanni, S. Corbianco, M. Dini, U. Carraro, N. Adami, S.
Zampieri, M. Carboncini, M. Giannetti, G. Cavallini, B. Rossi
U.O. Neuroriabilitazione, Azienda Ospedaliero-Universitaria Pisana (Pisa); Centro Interdipartimentale Biologia e Patologia dell’Invecchiamento, University of Pisa (Pisa); CirMyo - Lab. of Translational Myology, University of Padua (Padova); U.O. Neuroriab
Abstract
Background:Nitric oxide (NO) synthases (NOS) produce NO by oxidation of l-arginine (l-Arg). Excessive NO synthesis in the brain has cytotoxic effects1 and in mouse models NOS knockouts are protective after cerebrovascular injury. Asymmetric dimethylarginine (ADMA) is a well-known endogenous NOS inhibitor, that might be protective by limiting the toxic effect of high NO concentrations. Cell energy state can be determined by assaying concentration of the energy carrier molecule, adenosine-5"-triphosphate (ATP), that is an important determinant of cell death by apoptosis or necrosis: when ATP falls below a given threshold, apoptosis is activated3; a severe drop in ATP will result in cell necrosis. Metabolic conditions, such as trauma or stress, increase requirements for ATP, or reduce ATP regeneration, therefore decreasing the overall ATP available. Measurements of platelet ATP levels may be used for evaluation of the biochemical shift in body energy state. To our knowledge, there are no studies on differential platelet ATP concentrations and l-Arg/ADMA ratios in Aerobic Rehabilitation Protocol (ARP) vs Vibrational Training (VT) of Parkinson’s Disease (PD) patients.
Results:
Plasma l-Arg and ADMA concentrations were not significantly different between the groups at baseline, but ADMA levels were increased in all patients at the end of protocol (in VT: 3.1 ± 0.1 µM, p < 0.05; in ARP: 2.2 ± 0.2 µM, ns). Elevated ADMA plasma concentrations resulted in significantly decreased l-Arg/ADMA ratios in VT group compared to ARP one (< 20%). Significantly lower ATP levels were observed in ARP group platelets (0.016 ± 0.001 (mean ± SD) vs 0.029 ± 0.002 ATP fmols/platelet in VT group) at 6 months (p <0.001).
Schlagworte: Parkinson
GID: 2764; Letzte Änderung: 12.10.2011
